4/11/2023 0 Comments Chicken invaders 2 v2 60![]() ![]() Petri dish, a sterile scalpel was used to transfer mycelium to a 1.5 mL centrifuge tube and the genomic DNA was extracted with PrepMan Ultra Reagent (Applied Biosystems, CA, USA) following the manufacturer’s protocol. Once the fungal colonies had grown to the edge of 90 mm diam. Dried holotype specimens were conserved in the Herbarium of the Department of Plant Pathology, Agricultural College, Guizhou University (HGUP), while cultures were conserved in the Departmental Culture Collection (GUCC). All new taxa were registered in the Index Fungorum database ( (accessed on 13 October 2022). Morphological characteristics of the fungi were observed and photographed using a compound light microscope (Zeiss Scope 5) with an attached camera (AxioCam 208 color). After 12 h, the germination of conidium was observed, and they were then transferred to potato dextrose agar (PDA) and incubated at room temperature (28 ☌) for 10 days. Single conidia were picked off the leaves with a sterilized needle and placed on a drip board containing sterilized water. Abundant conidia were observed on the surface of the leaf spots examined using a dissecting microscope. To obtain pure cultures, leaf surfaces were disinfected according to Zhang et al. From 2021 to 2022, 94 samples were collected from orchards in six locations of Guizhou Province (Congjiang, Kaiyang, Longli, Luodian, Nayong and Wengan counties), including 10 host species (cherry, loquat, passionfruit, pitaya, plum, pomegranate, Rosa roxburghii, shaddock, tangerines and walnut). ![]()
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